Cryo-electron microscopy for integrative structural biochemistry
The course covers all typical steps of Single Particle Cryogenic Electron Microscopy (Cryo-M), from specimen preparation to atomic model building. Requirements of the sample for cryo-EM, major and most up-to-date software for performing single particle cryo-EM, as well as how Cryo-EM compares with and can be combined with other structural biology techniques are included. The course will also introduce other applications of cryogenic electron microscopy, such as cryo-electron tomography and electron diffraction of nano protein/compound crystals (microED).
After completing the course, the student is expected to be able to:
- Explain the theory behind Cryo-EM
- design a single particle cryo-EM project and identify possible pitfalls in the process
- perform a Cryo-EM experiment, from specimen preparation to model building of a target protein
- Show ability to analyse dynamic systems and use a combination of prediction methods, including AlphaFold, and manual model building.
Modules
The course consist of the following modules;
Module 1: Teori (theory), 5 hp
Module 2: Laboratory exercises (Laborationer), 2.5 hp
Teaching Format
Teaching consist of lectures, group instruction, seminars, exercises and laboratory sessions.
Assessment
The course is examined in the following manner:
Module 1 takes place through written exams, written reports and oral presentations.
Module 2 takes place through written reports and oral presentations.
Examiner
Marta Carroni
marta.carroni@dbb.su.se
Course Coordinator
Marta Carroni, email: marta.carroni@dbb.su.se
Chemistry Section & Student Affairs Office: chemistry@su.se
